Welcome to the department of microbiology infrared imaging for quantitative analysis core. Licor provides the complete solution for quantitative western blots with its data integrity bundle that includes standardized protocols, robust detection, validated reagents, online training, secure data analysis software, and ondemand support. Use of proteome profiler arrays with licor detection. Speakers todd holt technical support scientist licor biosciences. Reagents and consumables optimized for use on licor. The odyssey fc system is the only imaging system that offers superior chemiluminescent detection and the benefits of quantitative infrared western blot detection as well as the ability to document dna gels using the 600nm channel.
Image studio software can be used for quick acquisition and organization of images from most licor imaging systems, including the odyssey clx. Total protein analysis as a reliable loading control for. Licor biosciences introduces digiwest to accelerate targeted therapeutics development. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Jci insight exosomal tat protein activates latent hiv1. Odyssey digital imaging accessories pdf manual download. View online or download licor odyssey operators manual. Its technology allowed companies to manage multiple mobile operating systems at a detailed level, including functions such as inventory collection. Licor, odyssey, mpx, incell western, and irdye trademarks contained in the software product are. The xtreem logger xtract android app available for free download. It is an extremely simple and easytouse imaging software that simplifies new. Does anyone have experiences with both the licor odyssey scanner system, which uses. Image acquisition on odyssey clx with image studio software 5. Development and validation of an incell western for.
Odyssey software will be exhibiting at the i2i conference in pinehurst, nc on december 4th through 6th. In the face of changing data reporting guidelines, publish your results with confidence. Beadbased multiplexing assay unit with the ability to run up to 50 protein based assays such as elisas in a single reaction volume. Licor biosciences pioneering family of irdye infrared dyes are synthesized with reactive functional groups that enable easy covalent coupling to antibodies and other biomolecules. Themis sets the signal threshold for positive and negative. Manuals, application notes, data sheets, software upgrades and. Licor odyssey clx college of education and human sciences. Image studio is compatible with both mac and pc computers. Licor biosciences, a 44year old biotech hardware, software, and consumables company, grew out of an agronomy project, the development of light measurement devices for the study of photosynthesis.
Dysregulated foxo transcription factors in articular. The company produced games for the amiga and the nintendo entertainment system nes. Electronic technicians, working on an odyssey clx, our nearinfrared molecular imaging platform. Odyssey software was a computer game developer founded in 1987 in eastham, massachusetts by art v. Licor will not utilize such technical information in a form that personally identifies you. Read our licor data examples for mouse xl cytokine array to help with your experiment.
Try free image studio lite software for your western blot quantification. Support documents and contact information for licor products. Licor biosciences introduces digiwest to accelerate. Imaging system licor odyssey sa infrared imaging system. All images are acquired using the licor odyssey classic imager and associated image pro analysis software version 3. Odyssey software provided mobile device management and software development tools to enterprise companies either directly primarily through its athena product or through partner solutions. Licor biosciences is a leading manufacturer of nearinfrared and chemiluminescent imaging platforms, analysis software, and irdye infrared dye reagents for quantitative western blotting, small animal imaging, and dna fragment analysis. For more info, see the software specs you can use image studio lite software for basic western blot analysis, but it doesnt operate licor imagers or unicorns. Eddypro software is a powerful, free, and open source application for processing eddy covariance data. It has two lasers, which operate at 680 and 780nm, for the detection of infrared fluorescent dyes. Reagents and consumables optimized for use on licor instruments. For the complete list of video tutorials and support documents, visit image studio support.
Acquisition and analysis software for the odyssey clx. Licor lab instruments, equipment, and accessories biosurplus. Licor odyssey clx imaging system the lab world group. If you experience connection problems, wait several minutes and then click connect again. Pathway analysis with the incell western assay licor. If you have questions about how our administrative software solutions would work for your agency, stop. If you experience connection problems, wait several minutes and then.
Extended data figure 10 themis forms complexes with shp1 and shp2. The software searches for and discovers the odyssey clx imager on the same lan automatically. Glycoprotein detection with the odyssey infrared imaging system ingel western protocol oncell western platebased assay for targeted nearinfraredlabeled optical imaging agent development. You can use image studio lite software for basic western blot analysis, but it doesnt operate licor imagers or unicorns. Image studio software is compatible with most licor imaging systems, including the odyssey imaging systems, pearl imaging system, and cdigit blot. A guide to modern quantitative fluorescent western.
Find detailed information for all software versions. Image optimization most blots after scanning will require some adjustments in order to visualize the relevant portions of the images on the computer monitor. Odyssey clx infrared imaging system from licor biosciences. Odyssey clx manuals, application notes, software, and webinars. Odyssey imagers, pearl imagers, cdigit blot scanner, and aerius imager. Since the odyssey is a two channel instrument it can record data for either wavelength separately or both wavelengths simultaneously. Image studio lite is used for basic western blot analysis, but does not operate your licor instrument. There are certainly many different ways to both organize and retrieve. Odyssey software will be exhibiting at the open minds tii conference in philadelphia, pa on october 28th and 29th. Blots were washed in pbs and then water before acquisition on the licor odyssey.
Analytical facilities equipment school of biomedical. By using nir fluorescence detection, this imaging system greatly simplifies your western blot process by eliminating the need to incubate your substrate or wait for film. Odyssey clx infrared imaging system operators manual. You can use multiple colors at the same time to detect mouse and rabbit antibodies. When initial screen has loaded, click on file and select new from the dropdown menu. Accurate, replicable western blots with the odyssey clx licor. Read this entire manual before using the odyssey fc imager. Odyssey western blotting kit vi lt tbs 926340 odyssey western blotting kit viii lt tbs 92634017 pbs blocking buffer and pvdf kits 92632098,82932080. Odyssey clx application and technical notes show the versatility of the clx. Multiuser licenses are available so that you can use image studio on multiple computers. Odyssey software was founded in 1996 by mark gentile and originally focused on building software development tools. The licor odyssey infrared imager is an awesome instrument for western blot applications.
Find the best prices on used licor instruments, equipment, and accessories. Pair your odyssey clx imager with image studio software for streamlined. Image studio software support for licor imaging systems. Integrated intensity values k counts for each protein of interest were normalized to those of gapdh. The only western blot analysis software that guides you from validation through statistical analysis for reproducible results. Get truly quantitative data with the odyssey clx nearinfrared fluorescence.
Immunoblotting was performed using the snapid system of millipore zug, switzerland. The infrared imaging for quantitative analysis core was formed to give investigators access to the licor odyssey clx, an infrared imaging system for quantitative western blot analysis. Image studio is used for pearl imagers and cdigit blot scanner. Licor has developed unique data acquisition and flux computation, communication, and analysis software applications for every step in the eddy covariance research process. For conformity information, contact licor support at biosupport. Odyssey clx imager page 3 placement in the laboratory the odyssey clx infrared imaging system weighs approximately 33 kg 72 lbs. The odyssey software is essential to operate the odyssey classic loggers and manage the resulting data. Be confident in your western blotting data today and in the future. Licor biosciences, odyssey infrared imaging system b446. Image studio, image studio lite, and empiria studio software are used with odyssey imagers. The detection quality is excellent and the software is both easy to use and provides clear quantification.
Licor biosciences offers research solutions for robust western blots and targeted therapeutics development. Licor odyssey sa infrared imaging system biosurplus. This product model number 2800 is a cemarked product. Get consistent, reproducible digital images, without the hassles and unpredictability of film.
Manuals, software information, and webinars are also listed. Detection was achieved using the licor system, and band intensity was quantified with odyssey software licor, lincoln, nebraska, usa. Licor infrared imaging systems direct infrared detection is available on two different systems from licor biosciences the odyssey infrared imaging system and the aerius automated infrared imaging system. Odyssey to exhibit at i2i conference in north carolina. The odyssey clx uses the latest version of the licor image studio software. The odyssey fc imager is intended to be used by qualified personnel. If you are using windows 10, a separate driver must be installed to connect your cdigit blot scanner to image studio software via usb.
The instrument should be placed on a laboratory bench that is sufficiently sturdy to bear its weight. The research imaging facility rif provides approximately 2900 square feet of laboratory space dedicated to imaging and quantitative analyses. Image studio lite is western blot analysis software that is compatible with most image files and systems and is available for free download from the licor website. Pair your odyssey fc imager with image studio software for streamlined image acquisition and organization and with new empiria studio software for. Nearinfraredlabeled optical imaging agent development.
Image studio lite software is compatible with mac and windows. Membranes were scanned on the licor odyssey ir imaging instrument, and densitometric analysis was executed via the imagej software. The odyssey clx is the next generation multifunctional imaging platform that can provide a wide range of applications. Software notices andor terms and conditions are located in the licor odyssey fc imaging system. Additionally, i have used this for imaging my crystal violet stained plates. After providing your information, youll get an email with a link to download image studio lite software. Odyssey applications featuring irdye infrared dyes infrared dyes are a key performance advantage of the odyssey system. Western blotting, cellbased assays and more can benefit from licor infrared fluorescence technologies and imaging systems. Licor image studio software for accurate data quantification. High throughput multiplexing unit for quantification of multiple proteins in a single reaction via magnetic beads. Analyze your data right away and plan your next experiment. The same intuitive acquisition software is compatible with these licor imaging systems. Licor biosciences introduces digiwest to accelerate targeted therapeutics development news provided by.
Erk1, 2, and 5 expression and activation in dopaminergic. The licor odyssey clx imaging system gives your lab clear sharp digital results in as little as 6 minutes. The next time you connect, the software will automatically detect the odyssey clx imager. Used licor odyssey sa infrared imaging system 3014309.
With odyssey clx, you can get consistent, reproducible digital images, without the hassles and unpredictability of film. If you have questions about how our administrative software solutions would work for your agency, stop by our booth for more information. Determining the best blocking agent for each target will provide the best chance for successthe electrophoresis buffers maintain consistent ph levels during dna, rna, and other protein separation. This is necessary because the raw images consist of 16 bit tiff images. Trained researchers have 24hour access to the equipment, 7 days a week. Licor odyssey gel imaging scanner university of washington. Try empiria studio software today and enhance your odyssey workflow with the only western blot analysis software. Developed in collaboration with the eddy covariance research community, eddypro uses the most accepted and cited techniques to compute fullyprocessed fluxes of water vapor evapotranspiration, carbon dioxide, methane, other trace gases.
Topbottom before analysis with the odyssey software version 3. Licor biosciences introduces digiwest to accelerate targeted therapeutics development read this article along with other careers information, tips and advice on biospace researchers can identify therapeutic targets and functional effects of targeted therapeutics more quickly and efficiently as part of a complete solution now offered by li. Blot band intensity was measured using the licor odyssey software. The simple interface puts highquality data at your fingertips with a few simple clicks and without manual camera adjustments or multiple exposures. Staff provide education and training for equipment, technical support, protocol development, and contractual research projects. Odyssey software mobile device management wikipedia. Free western blot quantification software licor biosciences. Oct 14, 2010 protein transfer was performed using the dry system transfer iblot from invitrogen basel, switzerland. This simplification not only saves you productivity time, it also conserves water, and reduces the hazardous waste. Biosurplus has the most comprehensive and available list of used laboratory equipment online. View and download licor odyssey operators manual online. Partner with licor and let us help you take the next steps.
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